MD Anderson Cancer Center Sr Research Asst - GI Medical Oncology in Houston, Texas

Sr Research Asst - GI Medical Oncology

Location: United States, Texas, Houston, Houston (TX Med Ctr) at

Research at

GI Med Onc Rsch Department 710563

Requisition #: 109364

The ideal candidate will have experience with mouse models, tissue culture and molecular techniques.

The Sr Research Assistant provides supervision, special training to technical personnel and/or highly technical work in medical and related research projects. To be a critical member of the research lab and to assist the Principle Investigator and/or immediate supervisor in carrying out technical aspects of research projects.


Animal work: Under minimal supervision, tumor implantation subcutaneously or surgically, dose animals via oral gavage, intraperitoneal and intravenous injection. Measure tumor response via caliper and non-invasive imaging methods. Analyze the outcome of pharmacokinetic, pharmacodynamic and efficacy studies. Perform survival and non-survival animal surgeries for implantation of cell line and patient derive tumor fragments, collection of specimens for molecular characterization and ex vivo treatment using methods such as Ex vivo organotypic tissue cultures (EVOCs). Drug dosing techniques (such as oral gavage, IP and SQ) are required.

Tissue culture: Expand cell lines for xenograft and syngeneic tumor model implantation. Maintain numerous established cell lines using standard tissue culture techniques including; plating cells, determining cell counts and cell viability, transient and stable transfections, proliferation and functional assays, freezing cell lines properly to assure cell viability and aid in maintaining an organized cell repository. Perform ex vivo treatment of tumor fragments with therapeutic agents to by measuring viability, cell death and biomarkers of response.

Molecular techniques: Perform basic to intermediate molecular biology techniques such as DNA, RNA and protein extraction and measurement, DNA, RNA and protein gel electrophoresis (agarose and polycrylamide) hybridization techniques (Western blots, In situ Hybridization), real-time quantitative RT-PCR, recombinant DNA cloning and computer analysis of DNA sequences and PCR and primer design. Work will also involve the use antibodies in various immunostaining techniques and other indirect immunofluorescence techniques such as for flow cytometry and immunohistochemistry. 
Assistance and experience with lentiviral library screens is desired.

Record keeping: Maintain accurate, detailed records of experimental data and protocols in accordance with methods requested. This involves the use of computers to analyze, organize and plot data and for entry of data into databases.

Methods and Results Writing: The ability to provide concise and accurate write ups of methods and results as well as literature reviews is desired.

Assistance with lab personnel training: The capacity to assist with training of graduate students, fellows and post-doctoral trainees is desired.

Safety: Must maintain a clean and orderly work place. Must have the ability and willingness to observe and comply with all institutional rules and OSHA regulations regarding biohazard and radiation safety. Must have a working knowledge in the proper handling of hazardous chemicals such as phenol, ethidium bromide, acrylamide, and formamide. Must have a basic understanding of and willingness to work with radioisotopes.

General requirements: Must exhibit the following; a working knowledge of various laboratory equipment and apparatus, standard radiographic techniques and basic techniques for handling chemicals and accurately prepare media, solutions and reagents; an understanding of the basic scientific terminology and concepts central to cancer research; an ability to perform searches and retrieval of information both through the library and on line; a willingness to learn new techniques, to cooperate with other staff, students, fellows, visitors and collaborators, and to assist in other laboratory activities as needed.


Required: Bachelor's degree in one of the natural sciences or related field.

Preferred: Master's degree in one of the natural sciences or related field.


Required: Three years of experience in scientific or experimental research work. With preferred degree, one year of required experience

Preferred: Experience with mouse models, tissue culture and molecular techniques

It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law.